Isolated microspore culture of Brassica: An experimental tool for developmental studies and crop improvement
نویسندگان
چکیده
The technique of isolated microspore culture for the production of haploid plants is well standardized for many species of Brassica, some of which are important oilseed or vegetable crops. It offers an excellent experimental system for improvement of Brassica crops and study of cell differentiation during shift from gametophytic to sporophytic development. The haploids raised from microspores are of tremendous use in genetic improvement of brassicas through breeding, mutant induction and selection, and genetic engineering. The present review describes current status of the technology, the factors influencing the success of microspore culture, structural, biochemical and molecular changes occurring in the microspores triggered towards sporophytic development, and the progress made in practical applications of the microspore-derived plants.
منابع مشابه
Effects of Heat Shock and 2, 4-D Treatment on Morphological and Physiological Characteristics of Microspores and Microspore-Derived Doubled Haploid Plants in Brassica napus L.
Background: Stresses such as heat shock, starvation, or osmotic is essential to lead isolated microspores towards embryogenesis. Despite the effectiveness of stresses in embryogenesis, they exert adverse effects on metabolism and growth of the regenerated plants. Objectives: The effects of heat shock and 2,4-D treatment on total protein content of treated microspores, morphological and physiol...
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Induction of embryogenesis from isolated microspore cultures is a complex experimental system where microspores undergo dramatic changes in developmental fate. After ~40 years of application of electron microscopy to the study of the ultrastructural changes undergone by the induced microspore, there is still room for new discoveries. In this work, high pressure freezing and freeze substitution ...
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